Commercial inulinase from Aspergillus niger was immobilized in montmorillonite 
and then treated in pressurized propane and liquefied petroleum gas (LPG). 
Firstly, the effects of system pressure, exposure time, and depressurization 
rate, using propane and LPG, on enzymatic activity were evaluated through 
central composite design 2³. Residual activities of 145.1 and 148.5% were 
observed for LPG (30 bar, 6 h, and depressurization rate of 20 bar min⁻¹) and 
propane (270 bar, 1 h, and depressurization rate of 100 bar min⁻¹), 
respectively. The catalysts treated at these conditions in both fluids were then 
used for the production of fructooligosaccharides (FOS) using sucrose and inulin 
as substrates in aqueous and organic systems. The main objective of this step 
was to evaluate the yield and productivity in FOS, using alternatives for 
enhancing enzyme activity by means of pressurized fluids and also using low-cost 
supports for enzyme immobilization, aiming at obtaining a stable biocatalyst to 
be used for synthesis reactions. Yields of 18% were achieved using sucrose as 
substrate in aqueous medium, showing the potential of this procedure, hence 
suggesting a further optimization step to increase the process yield.