The fungicide agents are a key component in the fruits and vegetables 
production. The Iprodione residues are one of the pesticide more frequently 
found in food products. The available data about the cytotoxicity of iprodione 
and its metabolites are scarce and do not allow characterization of its 
genotoxic potential and define the risk assessment.The human larynx epidermoid 
carcinoma cell line (HEp-2) has been shown to be sensitive to the toxic effects 
of xenobiotics of different origin and have been often used in citotoxicity and 
genotoxicity studies. The purpose of this paper is to evaluate the induction of 
genotoxicity and the role of oxidative stress in HEp-2cell line by exposure to 
the IP. The MTT test for viability resulted in CL50 85.86 (77.05-95.68) μg/mL of 
Iprodione. On the basis of this result, we proceeded to expose the cells to the 
sublethal concentrations (below the CL50) during 24 h to analyze the mitotic 
index and nuclear division index in order to determine the subcytotoxic 
concentrations of IP which the genotoxicity was evaluated. The subcytotoxic 
concentrations of 7, 17, and 25 μg/mL IP induced aneugenic effects as 
micronuclei centromere positive whereas 17 μg/mL was a threshold for centromere 
negative micronuclei induction in HEp-2 cells. The abnormal mitosis was induced 
for exposition of Hep-2 cells to the three concentrations. According to the 
result obtained, citotoxicity and genotoxicity oxidative stress studies were 
performed in 1.5, 7.0, and 25 μg/mL of IP. The results showed that the GSH 
intracellular content, the SOD activity and the levels of oxidative damage of 
the proteins were affected lead to redox imbalance. The decreased in the SOD 
activity and protein oxidation were in according to the result obtained to 
genotoxicity, suggesting that different biological targets could be affected.