OBJECTIVE: To investigate the effect of a novel LMNA gene mutation E82K found in 
a Chinese family with dilated cardiomyopathy on cell cycle of HEK293 cells.
METHODS: (1) Human wild type full-length LMNA gene cDNA was subcloned into 
eukaryotic expression vector pTracer-CMV and point mutation was introduced into 
the cDNA. LMNA gene wild type and mutant E82K LMNA gene were transfected into 
HEK293 cells respectively and stable cell lines resistant to antibiotic were 
obtained 4 weeks later. (2) Cell cycle changes were analyzed by flow cytometry 
in HEK293 cells transfected with wild type and mutant E82K LMNA gene and empty 
vector in the presence of 0.8 mmol/L H(2)O(2).
RESULTS: Cell circle was arrested at G0/G1 phase in the cells transfected with 
mutated E82K LMNA gene and at G2/M phase in other cell groups in the presence of 
H(2)O(2).
CONCLUSION: Cell circle was arrested at G0/G1 phase in the cells transfected 
with E82K LMNA gene in the presence of H(2)O(2) in HEK293 cells.