MOTIVATION: Cellular mRNA levels originate from the combined action of multiple 
regulatory processes, which can be recapitulated by the rates of pre-mRNA 
synthesis, pre-mRNA processing and mRNA degradation. Recent experimental and 
computational advances set the basis to study these intertwined levels of 
regulation. Nevertheless, software for the comprehensive quantification of RNA 
dynamics is still lacking.
RESULTS: INSPEcT is an R package for the integrative analysis of RNA- and 
4sU-seq data to study the dynamics of transcriptional regulation. INSPEcT 
provides gene-level quantification of these rates, and a modeling framework to 
identify which of these regulatory processes are most likely to explain the 
observed mRNA and pre-mRNA concentrations. Software performance is tested on a 
synthetic dataset, instrumental to guide the choice of the modeling parameters 
and the experimental design.
AVAILABILITY AND IMPLEMENTATION: INSPEcT is submitted to Bioconductor and is 
currently available as Supplementary Additional File S1.
CONTACT: mattia.pelizzola@iit.it
SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics 
online.