High grade gliomas, including glioblastoma (GB), are devastating malignancies 
with very poor prognosis. Over the course of the last decade, there has been a 
failure to develop new treatments for GB. Reasons for this failure include the 
lack of validation of novel molecular targets, which are often characterized in 
animal models and directly transposed to human trials. Here we build on our 
previous findings, which describe how the multi-functional co-receptor 
Neuropilin-1 (NRP1) signals through glioma associated microglia/macrophages 
(GAMS) to promote murine glioma, and investigate NRP1 expression in human 
glioma. Clinical and gene expression data were obtained via The Cancer Genome 
Atlas (TCGA), and analyzed using R statistical software. Additionally, CIBERSORT 
in silico deconvolution was used to determine fractions of immune cell 
sub-populations within the gene expression datasets. We find that NRP1 
expression is correlated with poor prognosis, glioma grade, and associates with 
the mesenchymal GB subtype. In human GB, NRP1 expression is highly correlated 
with markers of monocytes/macrophages, as well as genes that contribute to the 
pro-tumorigenic phenotype of these cells.