RNA editing is a post-transcriptional modification of pre-mRNA that results in 
increased diversity in transcriptomes and proteomes. It occurs in a wide variety 
of eukaryotic organisms and in some viruses. One of the most common forms of 
pre-mRNA editing is A-to-I editing, in which adenosine is deaminated to inosine, 
which is read as guanosine during translation. This phenomenon has been observed 
in numerous transcripts, including the mammalian 5-HT(2C) receptor, which can be 
edited at five distinct sites. Methods used to date to quantify 5-HT(2C) 
receptor editing are labor-intensive, expensive and provide limited information 
regarding the relative abundance of 5-HT(2C) receptor editing variants. Here, we 
present a novel, ultra high-throughput method to quantify 5-HT(2C) receptor 
editing, compare it to a more conventional method, and use it to assess the 
effect of a range of genetic and pharmacologic manipulations on 5-HT(2C) 
editing. We conclude that this new method is powerful and economical, and we 
provide evidence that alterations in 5-HT(2C) editing appear to be a result of 
regional changes in brain activity, rather than a mechanism to normalize 
5-HT(2C) signaling.