Cas9/CRISPR has been reported to efficiently induce targeted gene disruption and 
homologous recombination in both prokaryotic and eukaryotic cells. Thus, we 
developed a Guide RNA Sequence Design Platform for the Cas9/CRISPR silencing 
system for model organisms. The platform is easy to use for gRNA design with 
input query sequences. It finds potential targets by PAM and ranks them 
according to factors including uniqueness, SNP, RNA secondary structure, and AT 
content. The platform allows users to upload and share their experimental 
results. In addition, most guide RNA sequences from published papers have been 
put into our database.