The use of liquid chromatography-electrospray ionization-tandem mass 
spectrometry (LC-ESI-MS(n)) for the glycoproteomic characterization of 
glycopeptides is a growing field of research. The N- and O-glycosylated peptides 
(N- and O-glycopeptides) analyzed typically originate from protease-digested 
glycoproteins where many of them are expected to be biomedically important. 
Examples of LC-MS(2) and MS(3) fragmentation strategies used to pursue glycan 
structure, peptide identity and attachment-site identification analyses of 
glycopeptides are described in this review. MS(2) spectra, using the CID and HCD 
fragmentation techniques of a complex biantennary N-glycopeptide and a core 1 
O-glycopeptide, representing two examples of commonly studied glycopeptide 
types, are presented. A few practical tips for accomplishing glycopeptide 
analysis using reversed-phase LC-MS(n) shotgun proteomics settings, together 
with references to the latest glycoproteomic studies, are presented.