In this study, we performed the first large-scale identification of N-terminal 
peptides from the green sulfur bacterium Chlorobaculum tepidum. Combined 
fractional diagonal chromatography (COFRADIC) was used to isolate protein 
N-terminal peptides from three different proteome preparations, and following 
LC-MS/MS analysis, over 621 different proteins were identified by their 
N-terminal peptides. Our data constitute the largest data set currently 
available for protein N-termini of prokaryotic photosynthetic organisms.