AIM: Evaluation of alterations of immune response regulation and possible risk 
of antenatal development of fetus in postvaccination period in pregnant women 
immunized against influenza A (H1N1).
MATERIALS AND METHODS: Women were vaccinated with MonoGrippol plus vaccine in 
the II trimester of physiological pregnancy. At certain intervals ofthe 
vaccination period (before the vaccination, 7 and 30 days after the vaccination) 
major biochemical markers in blood sera (alanine aminotransferase, aspartate 
aminotransferase, lactate dehydrogenase, alkaline phosphatase, creatinine, urea) 
and levels of key cytokines in spontaneous and stimulated test (IL-1alpha, 
IL-1RA, IL-2, IL-4, IL-10, IFNgamma, TNFalpha) were evaluated. Vaccination 
safety for the fetus and trophoblast development was evaluated by using human 
chorionic gonadotropin (HCG), alpha-fetoprotein (AFP) and trophoblasitc 
beta-1-glycoprotein (TBG) levels.
RESULTS: During vaccination in 13% of cases mild local reactions were noted, in 
26.1%--general systemic reactions in the form of weakness, dizziness and 
headaches. Levels of major biochemical markers at days 7 and 30 after the 
vaccination did not have any significant difference from the initial values (p > 
0.05). Cytokine levels in spontaneous and stimulated tests also did not change 
significantly. Markers of the course of pregnancy and fetus development (HCG, 
AFP and TBG) in the two groups observed had comparable values.
CONCLUSION: Vaccination of pregnant women against influenza A (H1N1) by Russian 
subunit formulation (MonoGrippol plus) showed reactogenicity comparable to 
control group by the level of influence on general metabolic and immunologic 
homeostasis and on the course of pregnancy, which is an evidence of its safety.