Wide hybridization is a one of the important techniques in plant breeding. Oat 
(Avena sativa L.) and pearl millet (Pennisetum glaucum L.) belong to different 
subfamilies of Poaceae. In generally, such distant relative species show 
uniparental chromosome elimination after successful fertilization. However, all 
seven pearl millet chromosomes are retained beside the genome of oat during 
embryogenesis. Hybrid seedlings develop, but show necrosis after light 
irradiation. Here, a detailed protocol for wide hybridization between oat and 
pearl millet is described.