To investigate the subtype distribution of human immunodeficiency virus-1(HIV-1) 
infection among men who have sex with men (MSM) in Zhengzhou, Henan Province, 
forty blood samples were collected from HIV-1 carriers, who acknowledged to have 
sex with men. The complete gag gene was amplified by RT-PCR and nested-PCR and 
sequenced. All sequences were edited by BioEdit and subtyped by genotyping 
software. Phylogenetic analysis of gag gene were then performed using the MEGA 
3.1 software, the gene distances were calculated by Distance program. There were 
three different HIV-1 subtypes including B, CRF01-AE and CRF07-BC present among 
twenty four MSMs in Zhengzhou. Genotyping results showed that 33.33% (8/24) were 
B, 41.67% (10/24) were CRF01-AE and 25% (6/24) were CRF07-BC, and subtype 
CRF01-AE had become the most prevalent HIV-1 subtype in Zhengzhou, Henan 
province. In conclusion, recombinant HIV-1 strains are circulating in Henan 
province and the epidemiology is complicated.