MOTIVATION: As cancer genomics initiatives move toward comprehensive 
identification of genetic alterations in cancer, attention is now turning to 
understanding how interactions among these genes lead to the acquisition of 
tumor hallmarks. Emerging pharmacological and clinical data suggest a highly 
promising role of cancer-specific protein-protein interactions (PPIs) as 
druggable cancer targets. However, large-scale experimental identification of 
cancer-related PPIs remains challenging, and currently available resources to 
explore oncogenic PPI networks are limited.
RESULTS: Recently, we have developed a PPI high-throughput screening platform to 
detect PPIs between cancer-associated proteins in the context of cancer cells. 
Here, we present the OncoPPi Portal, an interactive web resource that allows 
investigators to access, manipulate and interpret a high-quality cancer-focused 
network of PPIs experimentally detected in cancer cell lines. To facilitate 
prioritization of PPIs for further biological studies, this resource combines 
network connectivity analysis, mutual exclusivity analysis of genomic 
alterations, cellular co-localization of interacting proteins and domain-domain 
interactions. Estimates of PPI essentiality allow users to evaluate the 
functional impact of PPI disruption on cancer cell proliferation. Furthermore, 
connecting the OncoPPi network with the approved drugs and compounds in clinical 
trials enables discovery of new tumor dependencies to inform strategies to 
interrogate undruggable targets like tumor suppressors. The OncoPPi Portal 
serves as a resource for the cancer research community to facilitate discovery 
of cancer targets and therapeutic development.
AVAILABILITY AND IMPLEMENTATION: The OncoPPi Portal is available at 
http://oncoppi.emory.edu.
CONTACT: andrey.ivanov@emory.edu or hfu@emory.edu.