The pleiomorphic adenoma gene 1 (PLAG1) gene is activated in a subset of 
pleomorphic adenomas of the salivary gland by gene fusion. Germ‑line mutation in 
cylindromatosis (CYLD), a tumor suppressor gene, causes familial cylindromatosis 
and Brook‑Spiegler syndrome. In the present study, aberrations in PLAG1 and CYLD 
were investigated in adenoid cystic carcinoma (ACC) of the salivary gland. 
Reverse‑transcription PCR and PCR direct sequencing were performed to detect 
gene fusion of PLAG1 and mutation of CYLD in 34 ACC tissues. No PLAG1 fusion was 
detected in ACC. However, silent mutation of CYLD was detected in 2 cases of 
ACC, but no missense mutation was detected in ACC. These results suggest that 
PLAG1 and CYLD do not play a role in ACC tumorigenesis.