Holistic analysis of lipids is becoming increasingly popular in the life 
sciences. Recently, several interesting, mass spectrometry-based studies have 
been conducted, especially in plant biology. However, while great advancements 
have been made we are still far from detecting all the lipids species in an 
organism. In this study we developed an ultra performance liquid 
chromatography-based method using a high resolution, accurate mass, mass 
spectrometer for the comprehensive profiling of more than 260 polar and 
non-polar Arabidopsis thaliana leaf lipids. The method is fully compatible to 
the commonly used lipid extraction protocols and provides a viable alternative 
to the commonly used direct infusion-based shotgun lipidomics approaches. The 
whole process is described in detail and compared to alternative lipidomic 
approaches. Next to the developed method we also introduce an in-house developed 
database search software (GoBioSpace), which allows one to perform targeted or 
un-targeted lipidomic and metabolomic analysis on mass spectrometric data of 
every kind.