BACKGROUND: We investigated the effect of corn-derived biodiesel glycerol on 
microRNAs (miRNAs) and mRNAs, which play a central role in regulating cell 
survival, apoptosis and carcinogenesis.
MATERIALS AND METHODS: Inbred Balb/c mice were treated with purified glycerol 
from biodiesel for 24 hours. After administration, we determined the expressions 
of miR-21, miR-27a, miR-34a, miR-93, miR-143, miR-146a, miR-148a, miR-155, 
miR-196a, miR-203, miR-205, miR-221 and nuclear factor kappa-light-chain 
enhancer of activated B-cells-1 (Nfκb1), mitogen-activated protein kinase-8 
(Mapk8) and v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (K-ras) genes 
in the liver of mice.
RESULTS: We found a parallel altered expression of miRNAs and mRNAs in animals 
consuming biodiesel glycerol that compared to control mice; these alterations 
reached significant levels only in few cases.
CONCLUSION: Biodiesel glycerol presents no higher risk for carcinogenicity or 
toxicity.