Biochemical analysis of molecular interactions in specific genomic regions 
requires their isolation while retaining molecular interactions in vivo. Here, 
we report isolation of telomeres by engineered DNA-binding molecule-mediated 
chromatin immunoprecipitation (enChIP) using a transcription activator-like 
(TAL) protein recognizing telomere repeats. Telomeres recognized by the tagged 
TAL protein were immunoprecipitated with an antibody against the tag and 
subjected to identification of telomere-binding molecules. enChIP-mass 
spectrometry (enChIP-MS) targeting telomeres identified known and novel 
telomere-binding proteins. The data have been deposited to the ProteomeXchange 
with identifier PXD000461. In addition, we showed that RNA associated with 
telomeres could be isolated by enChIP. Identified telomere-binding molecules may 
play important roles in telomere biology. enChIP using TAL proteins would be a 
useful tool for biochemical analysis of specific genomic regions of interest.