Pre-mRNA splicing is a complex process involving combinatorial effects of cis- 
and trans-elements. Here, we focused on histone modifications as typical 
trans-regulatory elements and performed systematic analyses of associations 
between splicing patterns and histone modifications by using publicly available 
ChIP-Seq, mRNA-Seq, and exon-array data obtained in two human cell lines. We 
found that several types of histone modifications including H3K36me3 were 
associated with the inclusion or exclusion of alternative exons. Furthermore, we 
observed that the levels of H3K36me3 and H3K79me1 in the cell lines were well 
correlated with the differences in alternative splicing patterns between the 
cell lines.