Tetrodotoxin is a potent low weight marine toxin found in warm waters, 
especially of the Indian and Pacific Oceans. Intoxications are usually linked to 
the consumption of the puffer fish, although TTX was already detected in several 
different edible taxa. Benthic organisms such as mollusks and echinoderms, with 
different feeding habits, were collected monthly along the Portuguese coast from 
the summer of 2009 until the end of 2010. The extraction and analysis techniques 
were optimized and TTX and some analogues were detected for the first time in 
two intertidal gastropod species-Gibbula umbilicalis and Monodonta lineata by 
LC-MS/MS and UPLC-MS/MS. Although the levels are low, these findings suggest 
that monitoring of TTX and analogues in North Atlantic species should be 
implemented so as to detect potentially new toxin vectors and seasonal and/or 
geographical patterns.