Uric acid is the end-product of purine metabolism and a major antioxidant in 
humans. The concentrations of uric acid in plasma and urine are associated with 
various diseases and routinely measured in clinical and biomedical laboratories 
using enzymatic conversion and colorimetric measurement. In this study a 
hydrophilic interaction chromatographic (HILIC) method was developed for 
simultaneous determination of uric acid and creatinine, a biomarker of urine 
dilution and renal function, in human urine. Urine samples were pretreated by 
dilution, protein precipitation, centrifugation and filtration. Uric acid and 
creatinine were separated from other components in urine samples and quantified 
using HILIC chromatography. A linear relationship between the ratio of the peak 
area of the standards to that of the internal standard and the concentration of 
the standards was obtained for both uric acid and creatinine with the square of 
correlation coefficients >0.999 for both analytes. The detection limits were 
0.04 μg/mL for creatinine and 0.06 μg/mL for uric acid. The described HILIC 
method has proved to be simple, accurate, robust and reliable.