PURPOSE: Exposure to ionizing radiation causes damage to the genomic integrity 
and stability of the cell. Though a large number of molecules have been studied 
for their radioprotective capability, no single agent is available today that 
meets all the requirements of a good radiprotector. In this study, we have 
investigated a combination of Resveratrol (RSV) and 3,3'-Diindolyl methane (DIM) 
for its efficacy for radioprotection. It is our hypothesis that this combination 
that possesses less toxicity than synthetic compounds, free radical scavenging 
potential, and the capacity to interfere with the several of the signaling 
cascades that trigger damage to cell by ionizing radiation may possess good 
radioprotective capability.
MATERIALS AND METHODS: Mice were pre-treated with a combination of RSV and DIM 
and the 30-day mortality assay, endogenous antioxidant levels in intestinal 
mucosa, metaphase chromosomal aberrations, and micronuclei formation were 
assessed after exposed to ionizing radiation.
RESULTS: The dose modifying factor (DRF) obtained for RSV, DIM, and the 
combination is 1.15, 1.17, and 1.3, respectively. Pre-treatment of mice with the 
combination results in significant (***p = .001) protection of the endogenous 
antioxidant levels, chromosomal aberrations, micronuclei formation, after 
exposure to ionizing radiation.
CONCLUSIONS: Our findings suggest that pre-treatment with the combination of RSV 
and DIM protects effectively from the ionizing radiation-induced damage at the 
molecular, cellular, and tissue levels by counteracting both the direct and 
indirect effects.