BACKGROUND: Small GTPase proteins, including RhoA, RhoB, RhoC, Rac1, and cdc42, 
are important molecules for linking cell shape and cell-cycle progression 
because of their role in both cytoskeletal arrangements and mitogenic signaling. 
Over-expression of wild-type or constitutively active forms of RhoA has been 
shown to induce invasive behavior in non-invasive rat hepatoma cells in vitro. 
In addition, over-expression of RhoC has been found in melanoma cells with 
increasing metastatic activity as well as inflammatory breast cancer. These 
results indicate that overexpression of Rho proteins contributes to cancer cell 
invasion and metastasis. Rho GDP dissociation inhibitor 2 (RhoGDI2) was recently 
shown to act as a metastasis suppressor gene in bladder cancer. The purpose of 
this study was to clarify the clinical significance of this gene expression in 
patients with colorectal carcinoma.
METHODS: Fifty pairs of normal mucosa and cancer specimens obtained at the time 
of surgery from patients with colorectal cancer (CRC) were subjected to reverse 
transcription-polymerase chain reaction for RhoGDI2.
RESULTS: No patients with RhoGDI2-higher expression tumors had liver metastasis 
(0 in 8 cases); however, 33.3% (14 in 42 cases) of patients with RhoGDI2-lower 
expression tumors had liver metastasis. With regard to outcome in relation to 
RhoGDI2-positivity, RhoGDI2-higher expression tumors had a significant 
correlation with superior relapse-free survival (RFS) time as compared to 
RhoGDI2-lower expression tumors in stage III CRC (log-rank test, P < 0.05). 
Moreover, multivariate analysis indicated that RhoGDI2 was an independent 
prognostic factor for RFS.
CONCLUSION: RhoGDI2 is a novel predictor of RFS in patients with colorectal 
carcinoma.