Recent technological advances in RNA sequencing and analysis have allowed an 
increasingly thorough investigation of a previously unexplored class of 
transcripts, circular (circ)RNAs. Accumulating evidence suggests that circRNAs 
have unique functions which often rely on their association with microRNAs and 
RNA-binding proteins. Through these interactions, circRNAs have been implicated 
in major cellular processes and hence in the pathophysiology of a range of 
diseases. Here, we provide guidelines to consider when developing studies on 
circRNAs, including detecting and selecting the circRNAs, identifying their 
binding partners and sites of interaction, modulating circRNA levels, assessing 
copy numbers and stoichiometry, and addressing other points unique to circRNA 
analysis. This article is categorized under: Regulatory RNAs/RNAi/Riboswitches > 
Regulatory RNAs.