Laryngeal squamous cell carcinoma (LSCC) is a very common neoplasm of the head 
and neck in the world. Long noncoding RNAs play key roles in cell infiltration, 
fate, apoptosis, and invasion. However, the functional role and expression of 
LINC00339 remains unclear in LSCC. In this study, we showed that the expression 
level of LINC00339 was upregulated in LSCC tissues and cell lines. LINC00339 
silencing suppressed the proliferation, invasion, and epithelial-mesenchymal 
transition (EMT) progression of LSCC cells. In addition, we showed that 
LINC00339 acted as a sponge of miR-145, and LINC00339 silencing promoted the 
expression of miR-145 in Hep2 cell. Furthermore, the expression of miR-145 was 
lower in LSCC tissues than in their paired normal samples and the miR-145 
expression level was negatively correlated with LINC00339 expression in LSCC 
tissues. The knockdown of miR-145 promoted the proliferation, invasion, and EMT 
progression of LSCC cells. Finally, we indicated that LINC00339 silencing 
inhibited the proliferation, invasion, and EMT progression of LSCC cells by 
suppressing the miR-145 expression. These data suggested that LINC00339 acted as 
an oncogene in the development of LSCC, partly by regulating the miR-145 
expression.