Zinc finger proteins are a massive, diverse family of proteins that serve a wide 
variety of biological functions. However, the roles of them during meiosis are 
not yet clearly defined. Here, we report that Zfp207 localizes at the 
kinetochores during mouse oocyte meiotic maturation. Depletion of Zfp207 leads 
to a significantly higher proportion of impaired spindle organization and 
misaligned chromosomes in oocytes. This is coupled with the defective 
kinetochore-microtubule attachments, and resultantly increasing incidence of 
aneuploid metaphase II eggs. The precocious polar body extrusion and escape of 
metaphase I arrest induced by nocodazole treatment in Zfp207-depleted oocytes 
indicates that Zfp207 is essential for activation of SAC (Spindle Assembly 
Checkpoint) activity. Notably, we find that Zfp207 binds to Bub3 to form a 
complex and maintains its protein level in oocytes, and that overexpression of 
Bub3 is able to partially rescue the occurrence of aneuploid eggs in 
Zfp207-depleted oocytes. Collectively, we identify Zfp207 as a novel Bub3 
binding protein in oocytes which plays an important role in controlling meiotic 
chromosome alignment and SAC function.