Poliovirus protease 2A (2A(pro)) obstructs host gene expression by reprogramming 
transcriptional and post-transcriptional regulatory events during infection. 
Here we demonstrate that expression of 2A(pro) induces a selective 
nucleo-cytoplasm translocation of several important RNA binding proteins and 
splicing factors. Subcellular fractionation studies, together with 
immunofluorescence microscopy revealed an asymmetric distribution of HuR and 
TIA1/TIAR in 2A(pro) expressing cells, which modulates splicing of the human Fas 
exon 6. Consistent with this result, knockdown of HuR or overexpression of 
TIA1/TIAR, leads to Fas exon 6 inclusion in 2A(pro)-expressing cells. Therefore, 
poliovirus 2A(pro) can target alternative pre-mRNA splicing by regulating 
protein shuttling between the nucleus and the cytoplasm.