Despite the breadth of knowledge that exists regarding the function of long 
noncoding RNAs (lncRNAs) in biological phenomena, the role of lncRNAs in host 
antiviral responses is poorly understood. Here, we report that lncRNA#32 is 
associated with type I IFN signaling. The silencing of lncRNA#32 dramatically 
reduced the level of IFN-stimulated gene (ISG) expression, resulting in 
sensitivity to encephalomyocarditis virus (EMCV) infection. In contrast, the 
ectopic expression of lncRNA#32 significantly suppressed EMCV replication, 
suggesting that lncRNA#32 positively regulates the host antiviral response. We 
further demonstrated the suppressive function of lncRNA#32 in hepatitis B virus 
and hepatitis C virus infection. lncRNA#32 bound to activating transcription 
factor 2 (ATF2) and regulated ISG expression. Our results reveal a role for 
lncRNA#32 in host antiviral responses.