Epoxyeicosatrienoic acids (EETs) are the epoxidation products of arachidonic 
acid catalyzed by cytochrome P450 (CYP) epoxygenases, which possess multiple 
biological activities. In the present study, we aimed to explore the role and 
effects of CYP epoxygenases/EETs in wound healing in ob/ob mice. Full-thickness 
skin dorsal wounds were made on ob/ob mice and C57BL/6 control mice. The mRNA 
and protein expression of CYP epoxygenases were determined in granulation 
tissues of wounds. Effects of EETs on wound healing were evaluated. Inflammation 
and angiogenesis in wounds were also observed. Compared with C57BL/6 mice, the 
mRNA and protein expression of CYP2C65 and CYP2J6 in the granulation tissues in 
ob/ob mice were significantly reduced. 11,12-EET treatment significantly 
improved wound healing in ob/ob mice, whereas 14,15-EEZE, an EET antagonist, 
showed the opposite effect. 11,12-EET treatment decreased neutrophil and 
macrophage infiltration to the wound sites, resulting in reduced production of 
inflammatory cytokines, decreased MMP-9 expression, and increased collagen 
accumulation in the granulation tissues of ob/ob mice. In addition, 11,12-EET 
increased angiogenesis in the granulation tissues of wounds in ob/ob mice. These 
findings indicate that reduced expression of CYP epoxygenases may contribute to 
impaired diabetic wound healing, and exogenous EETs may improve diabetic wound 
healing by modulating inflammation and angiogenesis.