The motivation of this research is to establish a system of target genomic DNA 
capture and enrichment, which could be used in deep sequencing of target regions 
with next-generation sequencing. To design the 120 bp capture probes (baits) and 
prepare the SureSelect reagents, 2,414,977 bp human genomic sequence of 11,824 
exons in 1250 genes were submitted to the Agilent eArray platform and 
manufactured by Agilent. Two human genomic DNA samples were used and conducted 
the successive experiments for sequencing library construction: shearing 
fragmentation by sonication, blunt-ending and phosphorylation, adaptor ligation, 
150-200 bp fragments size selection, followed by hybridization with the baits, 
hybrid selection with magnetic beads, and PCR amplification. Prior to SOLiD 
sequencing reaction, the libraries were amplified with emulsion PCR and enriched 
with the P2 enrichment beads. The library samples were loaded to sequencing Chip 
for Work Flow Analysis (WFA) or sequencing running with default parameters. The 
results displayed that 46 509 baits were designed and synthesized for 11,147 
gene regions, and SureSelect capture probe regent was prepared. Real-time PCR 
showed the target enrichment efficiency up to 2(9) times with the SureSelect 
system. WFA revealed that the libraries were suitable for SOLiD Sequencing. The 
sequencing data revealed that 70% of the unique mapped sequence tags matched the 
target regions, and the average coverage of the target regions were above 
200-fold. All these demonstrated the feasibility of the established system of 
target genome sequence capture for next generation DNA sequencing.