Peyer's patch have been extensively studied as a major inductive site for 
mucosal immunity within the small intestine. The intestinal mucosa contains 
numerous dendritic cells, which induce either protective immunity to infectious 
agents or tolerance to innocuous antigens, including food and commensal 
bacteria. Although during the past few years, several subsets of human mucosal 
dendritic cells have been described, a precise characterization of the different 
mouse mucosal dendritic cells subpopulations remains to be achieved with regard 
to their phenotype and localization in Peyer's patch. In this report, we have 
investigated by immunofluorescence on cryosection and by flow cytometry, the 
phenotype and the localization of dendritic cells into Peyer's patch of C57Bl/6 
mouse intestine using dendritic cells markers. Positive and double staining for 
CD11c and BDCA-2, pDC/IPC, DC-LAMP, DC-SIGN, TLR8 and Langerin have been 
observed revealing new mouse intestinal DC subsets. This study provides new 
insight in the understanding of mucosal immune responses induced by natural 
processes as infections but also new perspectives for the evaluation of oral 
vaccines.