Human pigmentation is a polygenic trait which may be shaped by different kinds 
of gene-gene interactions. Recent studies have revealed that interactive effects 
between HERC2 and OCA2 may be responsible for blue eye colour determination in 
humans. Here we performed a population association study, examining important 
polymorphisms within the HERC2 and OCA2 genes. Furthermore, pooling these 
results with genotyping data for MC1R, ASIP and SLC45A2 obtained for the same 
population sample we also analysed potential genetic interactions affecting 
variation in eye, hair and skin colour. Our results confirmed the association of 
HERC2 rs12913832 with eye colour and showed that this SNP is also significantly 
associated with skin and hair colouration. It is also concluded that OCA2 
rs1800407 is independently associated with eye colour. Finally, using various 
approaches we were able to show that there is an interaction between MC1R and 
HERC2 in determination of skin and hair colour in the studied population sample.