In order to efficiently select aptamers that bind to and inhibit proteins, we 
developed a method that involves screening DNA aptamers based on their 
inhibitory activities using an evolution-mimicking algorithm after the 
pre-selection by SELEX. The value of this method was demonstrated by the 
identification of an inhibitor of Taq DNA polymerase in a unique single-stranded 
DNA library, which was expected to form a G-quartet structure. This method 
consists of selection via an inhibition assay, sequence shuffling, and mutation 
in silico. After six rounds of selection, the inhibitory activities of the 
aptamers had evolved significantly. This demonstrates the utility of this 
strategy for screening aptamers based on their inhibitory actions.