The characteristic AA(TT) sequence pattern of the nucleosome DNA derived earlier 
is used for prediction of nucleosome positions around splice junctions of 
eukaryotic genes. Two large datasets (2000 sequences each) were collected 
consisting of DNA segments with the exon/intron and intron/exon splice 
junctions, from various eukaryotic species. Positions of predicted nucleosomes 
near the junction sites were calculated. Those junctions which are found to 
belong to the nucleosomes, are located preferentially within a few base pairs 
from the midpoint of the nucleosome DNA. That is, obligatory GT- and AG-ends of 
the introns are more frequently located near the nucleosome dyad axis, within 
the best protected middle 10-15 base pairs of the nucleosome DNA. In addition, a 
tendency is observed for the strongest nucleosomes to form more often in the 
introns, in accordance with the hypothesis on the chromatin-organizing role of 
introns.