The Fusarium wilt caused by Fusarium oxysporum strains is the most devastating 
disease of cucumber, banana, and tomato. The biological control of this disease 
has become an attractive alternative to the chemical fungicides and other 
conventional control methods. In this review, the research trends and biological 
control efficiencies (BCE) of different microbial strains since 2000 are 
reviewed in detail, considering types of microbial genera, inoculum application 
methods, plant growth medium and conditions, inoculum application with 
amendments, and co-inoculation of different microbial strains and how those 
affect the BCE of Fusarium wilt. The data evaluation showed that the BCE of 
biocontrol agents was higher against the Fusarium wilt of cucumber compared to 
the Fusarium wilts of banana and tomato. Several biocontrol agents mainly 
Bacillus, Trichoderma, Pseudomonas, nonpathogenic Fusarium, and Penicillium 
strains were evaluated to control Fusarium wilt, but still this lethal disease 
could not be controlled completely. We have discussed different reasons of 
inconsistent results and recommendations for the betterment of BCE in the 
future. This review provides knowledge of the biotechnology of biological 
control of Fusarium wilt of cucumber, banana, and tomato in a nut shell that 
will provide researchers a beginning line to start and to organize and plan 
research for the future studies.