SAF-A/hnRNP U is an abundant nuclear protein that interacts specifically with 
nuclear matrix attachment region DNA (MAR) and RNA as a component of hnRNPs. 
SAF-A/hnRNP U was also shown to specifically bind mouse major satellite DNA 
(satMa). Antibodies against SAF-A and GFP-fusion constructs were used in the 
current work in order to trace SAF-A localization. In accordance with its 
diverse nucleic acid binding specificity, SAF-A was found to be localized in 
three different domains: outside the chromosomes, on the surface of the 
chromosome arms (probably MARs), and in the centromere region where it 
apparently binds specifically to the satMa. GFP-fusion constructs with different 
SAF-A/hnRNP U domains confirms the functional significance of the protein's 
functional domains in interphase cells. In telophase cells, the anti-SAF-A 
antibody signal appeared as a kind of network covering unfolded chromosomes.