The aim of tissue engineering is to produce functional three-dimensional (3D) 
tissue substitutes. Regarding native organ and tissue complexity, cell density 
and cell spatial 3D organization, which influence cell behavior and fate, are 
key parameters in tissue engineering. Laser-Assisted Bioprinting (LAB) allows 
one to print cells and liquid materials with a cell- or picoliter-level 
resolution. Thus, LAB seems to be an emerging and promising technology to 
fabricate tissue-like structures that have the physiological functionality of 
their native counterparts. This technology has additional advantages such as 
automation, reproducibility, and high throughput. It makes LAB compatible with 
the (industrial) fabrication of 3D constructs of physiologically relevant sizes. 
Here we present exhaustively the numerous steps that allow printing of viable 
cells with a well-preserved micrometer pattern. To facilitate the understanding 
of the whole cell patterning experiment using LAB, it is discussed in two parts: 
(1) preprocessing: laser set-up, bio-ink cartridge and bio-paper preparation, 
and pattern design; and (2) processing: bio-ink printing on the bio-paper.