Background: Extensive research has shown that long noncoding RNA (lncRNA) is 
involved in tumorigenesis, including hepatocellular carcinoma (HCC). The lncRNA 
LINC00339 was reported to regulate the development of lung cancer or breast 
cancer. However, whether LINC00339 participates in HCC progression remains 
unclear. Here, our results showed that LINC00339 was upregulated in HCC. 
Methods: qRT-PCR and in situ hybridization (ISH) was used to analyze LINC00339 
expression in tumor tissues and cell lines. CCK8 and colony formation assays 
were used to analyze cell proliferation. Transwell assay was used to analyze 
cell migration and invasion. Xenograft experiment was used to test tumor growth 
in vivo. Results: LINC00339 overexpression was correlated with an advanced 
stage, metastasis, and bad prognosis in HCC patients. Functional investigation 
showed that LINC00339 knockdown significantly suppressed HCC cell proliferation, 
migration, and invasion. Moreover, decreased LINC00339 expression inhibited HCC 
growth in vivo. Mechanistically, LINC00339 could interact with miR-1182 to 
promote SKA1 expression. We also demonstrated that SKA1 acted as an oncogene and 
SKA1 upregulation reversed the effect of LINC00339 silencing. Conclusion: Our 
results illustrated that the LINC00339/miR-1182/SKA1 axis plays an essential 
role in HCC progression.