During interphase in the budding yeast, Saccharomyces cerevisiae, centromeres 
are clustered near one pole of the nucleus as a rosette with the spindle pole 
body at its hub. Opposite to the centromeric pole is the nucleolus. Chromosome 
arms extend outwards from the centromeric pole and are preferentially directed 
towards the opposite pole. Centromere clustering is reduced by the ndc10 
mutation, which affects a kinetochore protein, and by the microtubule poison 
nocodazole. This suggests that clustering is actively maintained or enforced by 
the association of centromeres with microtubules throughout interphase. Unlike 
the Rabl-orientation known from many higher eukaryotes, centromere clustering in 
yeast is not only a relic of anaphase chromosome polarization, because it can be 
reconstituted without the passage of cells through anaphase. Within the rosette, 
homologous centromeres are not arranged in a particular order that would suggest 
somatic pairing or genome separation.