Leguminous plants open their leaves during the daytime and close them at night 
as if sleeping, a type of movement that follows circadian rhythms, and is known 
as nyctinastic movement. This phenomenon is controlled by two endogenous 
bioactive substances that exhibit opposing activities: Leaf-Opening Factor 
(LOF), which opens the leaves, and Leaf-Closing Factor (LCF), which closes them. 
The authors have carried out chemical biological research using these bioactive 
substances as molecular probes in order to clarify the mechanisms of nyctinastic 
movement. Here, we report on the detection and identification of the target 
proteins of these compounds using original methodology.