The diverse arrays of proteins secreted by senescent cells have been described 
to influence aging and to have both pro-tumorigenic and anti-tumorigenic 
influences on the surrounding microenvironment. Further characterization of 
these proteins, known as the senescence-associated secretory phenotype (SASP), 
and their regulators is required to understand and further manipulate such 
activities. The use of high-throughput technology allows us to obtain visual and 
quantitative data on a large number of samples quickly and easily. Not only is 
this an invaluable tool for conducting large-scale RNAi or compound screenings, 
but also allows rapid validation of candidates of interest. Here, we describe 
how we use the Widefield High-Content Analysis Systems to characterize the 
phenotypes of cells following modulation by potential regulators of 
Oncogene-Induced Senescence (OIS) by measuring numerous markers of senescence, 
including the SASP. This approach can be also used to screen for siRNA able to 
perturb the expression of SASP components during OIS.