Molecular dynamics (MD) simulation methods have seen significant improvement 
since their inception in the late 1950s. Constraints of simulation size and 
duration that once impeded the field have lessened with the advent of better 
algorithms, faster processors, and parallel computing. With newer techniques and 
hardware available, MD simulations of more biologically relevant timescales can 
now sample a broader range of conformational and dynamical changes including 
rare events. One concern in the literature has been under which circumstances it 
is sufficient to perform many shorter timescale simulations and under which 
circumstances fewer longer simulations are necessary. Herein, our simulations of 
the zinc finger NEMO (2JVX) using multiple simulations of length 15, 30, 1000, 
and 3000 ns are analyzed to provide clarity on this point.