Gan et al. (Proteomics 2013, 13, 3117-3123) described a new "macropore" protocol 
for effective protein digestion by trypsin suitable for a wide range of pH 
including acidic pH. It was effective not only in experiments with solutions of 
a model protein (myoglobin), but also with a subfraction of rat liver cytosol. 
This significantly simplifies and accelerates protein digestion procedures for 
subsequent MS. However, further studies are needed to find limits of 
experimental applicability of the described protocol in proteomics.