DNA methyltransferase2 (DNMT2) is always deemed to be enigmatic, because it 
contains highly conserved DNA methyltransferase motifs but lacks the DNA 
methylation catalytic capability. Here we show that Arabidopsis DNA 
methyltransferase2 (AtDNMT2) is localized in nucleus and associates with histone 
deacetylation. Bimolecular fluorescence complementation and pull-down assays 
show AtDNMT2 interacts with type-2 histone deacetylases (AtHD2s), a unique type 
of histone deacetylase family in plants. Through analyzing the expression of 
AtDNMT2: ss-glucuronidase (GUS) fusion protein, we demonstrate that AtDNMT2 has 
the ability to repress gene expression at transcription level. Meanwhile, the 
expression of AtDNMT2 gene is altered in athd2c mutant plants. We propose that 
AtDNMT2 possibly involves in the activity of histone deacetylation and plant 
epigenetic regulatory network.