We determined the times when the nuclear membrane, nuclear pore complex (NPC) 
components, and nuclear import function were recovered during telophase in 
living HeLa cells. Simultaneous observation of fluorescently-labeled NLS-bearing 
proteins, lamin B receptor (LBR)-GFP, and Hoechst33342-stained chromosomes 
revealed that nuclear membranes reassembled around chromosomes by 5 minutes 
after the onset of anaphase (early telophase) whereas nuclear import function 
was recovered later, at 8 minutes. GFP-tagged emerin also accumulated on 
chromosomes 5 minutes after the onset of anaphase. Interestingly, emerin and LBR 
initially accumulated at distinct, separate locations, but then became uniform 8 
minutes after the onset of anaphase, concurrent with the recovery of nuclear 
import function. We further determined the timing of NPC assembly by 
immunofluorescence staining of cells fixed at precise times after the onset of 
anaphase. Taken together, these results showed that emerin, LBR, and several NPC 
components (RanBP2, Nup153, p62), but not Tpr, reconstitute around chromosomes 
very early in telophase prior to the recovery of nuclear import activity.