PIM kinases are implicated in variety of cancers by promoting cell survival and 
proliferation and are targets of interest for therapeutic intervention. We have 
identified a low-nanomolar pan-PIM inhibitor (PIM1/2/3 potency 5:14:2nM) using 
structure based modeling. The crystal structure of this compound with PIM1 
confirmed the predicted binding mode and protein-ligand interactions except 
those in the acidic ribose pocket. We show the SAR suggesting the importance of 
having a hydrogen bond donor in this pocket for inhibiting PIM2; however, this 
interaction is not important for inhibiting PIM1 or PIM3. In addition, we report 
the discovery of a new class of PIM inhibitors by using computational de novo 
design tool implemented in MOE software (Chemical Computing Group). These 
inhibitors have a different interaction profile.