The auxin-inducible degron (AID) system allows the rapid and reversible 
proteolysis of proteins of interest, and enables the generation of conditional 
mutants of budding yeast. The construction of budding yeast AID mutants is 
simple, and the effect of depletion of essential proteins on proliferation can 
be confirmed by analyzing their phenotype. In this protocol, we describe a 
procedure to generate AID mutants of budding yeast via a simple transformation 
using PCR-amplified DNA. We also describe methods to confirm the depletion of 
proteins of interest that are required for proliferation by serial-dilution and 
liquid-culture assays.