Chargaff's rule of intra-strand parity (ISP) between complementary 
mono/oligonucleotides in chromosomes is well established in the scientific 
literature. Although a large numbers of papers have been published citing works 
and discussions on ISP in the genomic era, scientists are yet to find all the 
factors responsible for such a universal phenomenon in the chromosomes. In the 
present work, we have tried to address the issue from a new perspective, which 
is a parallel feature to ISP. The compositional abundance values of 
mono/oligonucleotides were determined in all non-overlapping sub-chromosomal 
regions of specific size. Also the frequency distributions of the 
mono/oligonucleotides among the regions were compared using the 
Kolmogorov-Smirnov test. Interestingly, the frequency distributions between the 
complementary mono/oligonucleotides revealed statistical similarity, which we 
named as intra-strand frequency distribution parity (ISFDP). ISFDP was observed 
as a general feature in chromosomes of bacteria, archaea and eukaryotes. 
Violation of ISFDP was also observed in several chromosomes. Chromosomes of 
different strains belonging a species in bacteria/archaea (Haemophilus 
influenza, Xylella fastidiosa etc.) and chromosomes of a eukaryote are found to 
be different among each other with respect to ISFDP violation. ISFDP correlates 
weakly with ISP in chromosomes suggesting that the latter one is not entirely 
responsible for the former. Asymmetry of replication topography and composition 
of forward-encoded sequences between the strands in chromosomes are found to be 
insufficient to explain the ISFDP feature in all chromosomes. This suggests that 
multiple factors in chromosomes are responsible for establishing ISFDP.