Cancer stem cells (CSCs) play a central role in the development of breast 
cancer. The canonical Wnt/β-catenin signal pathway is critical for maintaining 
CSCs characteristics. Diallyl trisulfide (DATS), a natural organosulfur compound 
from the garlic, exhibits effective antitumor properties. However, the role of 
DATS in regulating breast CSCs activity and the underlying molecular mechanisms 
remain obscure. In the present study, we reported that DATS efficiently 
inhibited the viability of breast CSCs as evidenced by reducing turmorspheres 
formation, decreasing the expression of breast CSCs markers (CD44, ALDH1A1, 
Nanog, and Oct4), as well as inhibiting proliferation and inducing apoptosis. 
Furthermore, we showed that DATS downregulated the activity of Wnt/β-catenin 
pathway, while LiCl-triggered Wnt/β-catenin activation diminished DATS 
inhibition on breast CSCs. Taken together, our results illustrated that DATS 
suppressed breast CSCs through inhibiting Wnt/β-catenin pathway activation. 
These novel findings could provide new insights into the molecular mechanisms of 
breast CSCs regulation as well as its target intervention and might provide new 
strategies for preventing and treating breast cancers.