BACKGROUND: Zika virus (ZIKV; genus Flavivirus, family Flaviviridae) is 
maintained in a zoonotic cycle between arboreal Aedes spp. mosquitoes and 
nonhuman primates in African and Asian forests. Spillover into humans has been 
documented in both regions and the virus is currently responsible for a large 
outbreak in French Polynesia. ZIKV amplifications are frequent in southeastern 
Senegal but little is known about their seasonal and spatial dynamics. The aim 
of this paper is to describe the spatio-temporal patterns of the 2011 ZIKV 
amplification in southeastern Senegal.
METHODOLOGY/FINDINGS: Mosquitoes were collected monthly from April to December 
2011 except during July. Each evening from 18:00 to 21:00 hrs landing 
collections were performed by teams of 3 persons working simultaneously in 
forest (canopy and ground), savannah, agriculture, village (indoor and outdoor) 
and barren land cover sites. Mosquitoes were tested for virus infection by virus 
isolation and RT-PCR. ZIKV was detected in 31 of the 1,700 mosquito pools 
(11,247 mosquitoes) tested: Ae. furcifer (5), Ae. luteocephalus (5), Ae. 
africanus (5), Ae. vittatus (3), Ae. taylori, Ae. dalzieli, Ae. hirsutus and Ae. 
metallicus (2 each) and Ae. aegypti, Ae. unilinaetus, Ma. uniformis, Cx. 
perfuscus and An. coustani (1 pool each) collected in June (3), September (10), 
October (11), November (6) and December (1). ZIKV was detected from mosquitoes 
collected in all land cover classes except indoor locations within villages. The 
virus was detected in only one of the ten villages investigated.
CONCLUSIONS/SIGNIFICANCE: This ZIKV amplification was widespread in the Kédougou 
area, involved several mosquito species as probable vectors, and encompassed all 
investigated land cover classes except indoor locations within villages. Aedes 
furcifer males and Aedes vittatus were found infected within a village, thus 
these species are probably involved in the transmission of Zika virus to humans 
in this environment.