The social amoeba Dictyostelium discoideum is already known as a model organism 
for a variety of cellular and molecular studies. Now that the genome sequencing 
project has been completed and different tools with which to overexpress or 
knock out genes are available, this species has also moved into the spotlight of 
functional genomics studies. Consequently, this genomic sequence information can 
now be exploited to realize D. discoideum proteomics projects. Here, we present 
validated protocols adapted for analysis of the D. discoideum proteome. The 
workflow described in this chapter comprises two-dimensional polyacrylamide gel 
electrophoresis for protein separation and peptide mass fingerprint 
(matrix-assisted laser desorption/ionization time-of-flight mass spectrometry) 
for protein identification.