Previous studies demonstrated that massive induction of transcriptional 
readthrough generates downstream of gene-containing transcripts (DoGs) in cells 
under stress condition. Here, we analyzed TSS-seq (transcription start site 
sequencing) data from the DBTSS database. We investigated TSS tags at the end of 
gene for all pan-stress and untreated-cell DoGs, in comparison with 
expression-matched non-DoGs. We observed significantly more TSS tags at the end 
of pan-stress and untreated-cell DoG genes than non-DoG genes, even though their 
TSS tags in the promoter is the same. Importantly, the median value of TSS tags 
at gene end normalized to gene promoter is significantly higher than the median 
expression ratio of short DoG to host gene and of long DoG to host gene. Our 
results indicate that downstream overlapping long non-coding RNAs derived from 
the TSS at the gene end may be an important source of DoGs.