SUMMARY: Here we introduce Lipid Mini-On, an open-source tool that performs 
lipid enrichment analyses and visualizations of lipidomics data. Lipid Mini-On 
uses a text-mining process to bin individual lipid names into multiple lipid 
ontology groups based on the classification (e.g. LipidMaps) and other 
characteristics, such as chain length. Lipid Mini-On provides users with the 
capability to conduct enrichment analysis of the lipid ontology terms using a 
Shiny app with options of five statistical approaches. Lipid classes can be 
added to customize the user's database and remain updated as new lipid classes 
are discovered. Visualization of results is available for all classification 
options (e.g. lipid subclass and individual fatty acid chains). Results are also 
visualized through an editable network of relationships between the individual 
lipids and their associated lipid ontology terms. The utility of the tool is 
demonstrated using biological (e.g. human lung endothelial cells) and 
environmental (e.g. peat soil) samples.
AVAILABILITY AND IMPLEMENTATION: Rodin (R package: 
https://github.com/PNNL-Comp-Mass-Spec/Rodin), Lipid Mini-On Shiny app 
(https://github.com/PNNL-Comp-Mass-Spec/LipidMiniOn) and Lipid Mini-On online 
tool (https://omicstools.pnnl.gov/shiny/lipid-mini-on/).
SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics 
online.