A novel series of carbamate-linked cationic lipids containing hydroxyl headgroup 
were synthesized and included in formulations for transfection assays. The 
DNA-lipid complexes were characterized for their ability to bind DNA, their 
size, ζ-potential and cytotoxicity. Compared with our previously reported 
cationic transfection lipid DDCDMA lacking the hydroxyl group and the 
commercially available, these cationic liposomes exhibited relatively higher 
transfection efficiency.