The study of plant parasitic nematodes such as Meloidogyne spp. and their 
interactions with phytopathogenic bacteria remains underexplored. One of the 
challenges towards establishing such interactions is the dependence on symptom 
development as a measure of interaction. In this study, mCherry was employed as 
a reporter protein to investigate the interaction between the soft rot 
Enterobacteriaceae (SRE) Pectobacterium carotovorum subsp. brasiliensis (Pcb) 
and root-knot nematode (M. incognita). Pectobacterium carotovorum subsp. 
brasiliensis was transformed with pMP7604 generating Pcb_mCherry strain. This 
strain was shown to attach to the surface coat of M.incognita J2 at the optimum 
temperature of 28°C. This suggests that RKN juveniles may play a role in 
disseminating Pcb in soils that are heavily infested with Pcb. The presence of 
RKN juveniles was shown to play a role in introducing Pcb_mCherry into potato 
tubers potentially acting as a source of latent tuber infections.