Biotechnologically produced itaconic acid (IA) is a promising organic acid with 
a wide range of applications and the potential to open up new application fields 
in the area of polymer chemistry, pharmacy, and agriculture. In this study, a 
systematic process optimization was performed with an own isolated strain of 
Aspergillus terreus and transferred from a 250-mL to a 15-L scale. An IA 
concentration of 86.2 g/L was achieved within 7 days with an overall 
productivity of 0.51 g/(L h), a maximum productivity of 1.2 g/(L h), and a yield 
of 86 mol%. A cultivation of other well-known A. terreus strains with the 
developed process showed no significant differences. Based on this, a process is 
developed providing a high final IA concentration independent of the used strain 
combined with high reproducibility.