Eukaryotic genomes are extensively transcribed, generating many different RNAs 
with no known function. We have constructed 1502 molecular barcoded ncRNA gene 
deletion strains encompassing 443 ncRNAs in the yeast Saccharomyces cerevisiae 
as tools for ncRNA functional analysis. This resource includes deletions of 
small nuclear RNAs (snRNAs), transfer RNAs (tRNAs), small nucleolar RNAs 
(snoRNAs), and other annotated ncRNAs as well as the more recently identified 
stable unannotated transcripts (SUTs) and cryptic unstable transcripts (CUTs) 
whose functions are largely unknown. Specifically, deletions have been 
constructed for ncRNAs found in the intergenic regions, not overlapping genes or 
their promoters (i.e., at least 200 bp minimum distance from the closest gene 
start codon). The deletion strains carry molecular barcodes designed to be 
complementary with the protein gene deletion collection enabling parallel 
analysis experiments. These strains will be useful for the numerous genomic and 
molecular techniques that utilize deletion strains, including genome-wide 
phenotypic screens under different growth conditions, pooled chemogenomic 
screens with drugs or chemicals, synthetic genetic array analysis to uncover 
novel genetic interactions, and synthetic dosage lethality screens to analyze 
gene dosage. Overall, we created a valuable resource for the RNA community and 
for future ncRNA research.