This unit describes how to use the gene-finding programs GeneMark.hmm-E and 
GeneMark-ES for finding protein-coding genes in the genomic DNA of eukaryotic 
organisms. These bioinformatics tools have been demonstrated to have 
state-of-the-art accuracy for many fungal, plant, and animal genomes, and have 
frequently been used for gene annotation in novel genomic sequences. An 
additional advantage of GeneMark-ES is that the problem of algorithm 
parameterization is solved automatically, with parameters estimated by iterative 
self-training (unsupervised training).