The proteomes of the severe acute respiratory syndrome-associated coronavirus 
(SARS-CoV) and its infected Vero E6 cells were detected in the present study. 
The cytosol and nucleus fractions of virus-infected cells as well as the crude 
virions were analyzed either by one-dimensional electrophoresis followed by 
ESI-MS/MS identification or by shotgun strategy with two-dimensional liquid 
chromatography-ESI-MS/MS. For the first time, all of the four predicted 
structural proteins of SARS-CoV were identified, including S (Spike), M 
(Membrane), N (Nucleocapsid), and E (Envolope) proteins. In addition, a novel 
phosphorylated site of M protein was observed. The combination of these gel-base 
and non-gel methods provides fast and complimentary approaches to SARS-CoV 
proteome and can be widely used in the analysis of other viruses.