Recent development in the proteomic technologies offers new perspectives in 
circadian biology and in particular the possibility to study post-translational 
modifications such as phosphorylation and acetylation. Applying in vivo 
proteomics on whole liver or on nuclear extracts, we were able to characterize 
the rhythmic liver proteome with unprecedented coverage. It allows the 
characterization of new rhythmic processes such as protein secretion, ribosome 
biogenesis, DNA repair, and polyploidy. In addition, the analysis of rhythmic 
post-translational modifications helps to understand the signal pathways 
involved and their consequences on hepatic metabolism.