We have analyzed the interference of antitumoral drugs acting through the 
inhibition of DNA topoisomerase II on the human HeLa cell metabolism. Different 
compounds characterized by a diverse mechanism of action have been used, namely 
m-amsacrine, an intercalative drug, etoposide, which does not intercalate DNA, 
and suramin, which exerts its effect through an unknown mechanism. In HeLa cells 
treated with increasing doses of these drugs, we have examined cell viability 
and DNA synthesis capacity, and we have evaluated topoisomerase II activity. 
Cellular morphology and DNA integrity have been studied in order to characterize 
the mechanism of cell death. The results we have obtained clearly indicate that 
topoisomerase II poisons induce cell death by apoptosis. These observations 
suggest a role of the inhibition of topoisomerase II activity in the apoptotic 
program.