Argonaute 2 (AGO2) is an indispensable component of the RNA-induced silencing 
complex, operating at the translational or posttranscriptional level. It is 
compartmentalized into structures such as GW- and P-bodies, stress granules and 
adherens junctions as well as the midbody. Here we show using 
immunofluorescence, image and bioinformatic analysis and cytogenetics that AGO2 
also resides in membrane protrusions such as open- and close-ended tubes. The 
latter are cytokinetic bridges where AGO2 colocalizes at the midbody arms with 
cytoskeletal components such as α-Τubulin and Aurora B, and various kinases. 
AGO2, phosphorylated on serine 387, is located together with Dicer at the 
midbody ring in a manner dependent on p38 MAPK activity. We further show that 
AGO2 is stress sensitive and important to ensure the proper chromosome 
segregation and cytokinetic fidelity. We suggest that AGO2 is part of a 
regulatory mechanism triggered by cytokinetic stress to generate the appropriate 
micro-environment for local transcript homeostasis.