In the nucleus HuR binds to mRNAs containing adenylate-uridylate rich elements 
in the 3'-untranslated region. HuR may influence expression of its ligand mRNA 
through regulation of polyadenylation, translocation of the message to the 
cytosol, stabilization of the mRNA and/or altering its translational efficiency. 
Suppression of HuR using siRNA resulted in an attenuation of the 3T3-L1 
differentiation program, consistent with HuR control of the expression of mRNA 
ligand(s) critical to the differentiation process. In the present study, we 
begin to identify mRNA ligands of HuR whose regulated expression is necessary 
for adipogenesis.