Employing a combination of reverse transcription-polymerase chain reaction 
(RT-PCR) and rapid amplification of cDNA ends (RACE) techniques, the complete 
coding sequence of cDNA for the equine SRY gene was determined. We also 
attempted to make clear whether the equine SRY gene transcript is expressed in 
the adult testis, and whether the type of transcript is expressed as linear or 
circular RNA. As a result, in total a 1420 bp cDNA sequence was determined. 
Accomplishment of 3' RACE infers that equine SRY gene was expressed as a linear 
RNA transcript in testicular tissue just after puberty, in contrast to the 
situation in mice.