This chapter describes sequencing-based methods for profiling dynamic changes in 
DNA accessibility and gene expression in Saccharomyces cerevisiae. Assay for 
transposase-accessible chromatin with high-throughput sequencing (ATAC-Seq) is a 
powerful technique for identifying nucleosome-free regions of the genome. 
Combining ATAC-Seq with RNA Sequencing (RNA-Seq) is a rapid approach for 
studying the relationship between genome structure and changes in global 
patterns of gene expression from a single experiment. A laboratory protocol is 
presented for these methods as well as examples of typical results and 
visualizations.