The Chromosome 16 Consortium forms part of the Human Proteome Project that aims 
to develop an entire map of the proteins encoded by the human genome following a 
chromosome-centric strategy (C-HPP) to make progress in the understanding of 
human biology in health and disease (B/D-HPP). A Spanish consortium of 16 
laboratories was organized into five working groups: Protein/Antibody 
microarrays, protein expression and Peptide Standard, S/MRM, Protein Sequencing, 
Bioinformatics and Clinical healthcare, and Biobanking. The project is conceived 
on a multicenter configuration, assuming the standards and integration 
procedures already available in ProteoRed-ISCIII, which is encompassed within 
HUPO initiatives. The products of the 870 protein coding genes in chromosome 16 
were analyzed in Jurkat T lymphocyte cells, MCF-7 epithelial cells, and the 
CCD18 fibroblast cell line as it is theoretically expected that most chromosome 
16 protein coding genes are expressed in at least one of these. The 
transcriptome and proteome of these cell lines was studied using gene expression 
microarray and shotgun proteomics approaches, indicating an ample coverage of 
chromosome 16. With regard to the B/D section, the main research areas have been 
adopted and a biobanking initiative has been designed to optimize methods for 
sample collection, management, and storage under normalized conditions and to 
define QC standards. The general strategy of the Chr-16 HPP and the current 
state of the different initiatives are discussed.