Our prior study had shown that resveratrol was a potent cardioprotective agent 
in rats with myocardial infarction (MI). In this study, we further evaluated the 
mechanism of cardioprotection of resveratrol by proteomic analysis. After 
permanent ligation of the left anterior descending artery under isoflurane 
anesthesia, surviving rats were randomly allocated to three groups and treated 
with resveratrol at 1 mg/kg/day (MI/R group), or vehicles (sham group and MI 
group) once daily for four weeks. In proteomic analysis, the MI group showed 
decreased expression of adenylate kinase 1 (AK1) and mitochondrial 
NADP⁺-dependent isocitrate dehydrogenase (IDPm) after MI compared with the sham 
group. These variations were reversed by resveratrol in the MI/R group. 
Validation with Western blot and immunohistochemical analyses showed similar 
trends in protein expression profiling. Our studies suggest that the beneficial 
effects of resveratrol on ventricular modeling may be due to increased 
expression of AK1 and IDPm, which have been known to increase myocardial 
energetic efficiency and reduce reactive oxygen species-mediated damage, 
respectively.