The detection and quantification of methylated RNA can be beneficial to 
understand certain cellular regulation processes such as transcriptional 
modulation of gene expression, immune response, or epigenetic alterations. 
Therefore, it is necessary to have methods available, which are extremely 
sensitive and accurate, for instance liquid chromatography-tandem mass 
spectrometry (LC-MS/MS). Here, we describe the preparation of RNA samples by 
enzymatic hydrolysis and the subsequent analysis of ribonucleosides by LC-MS/MS 
via NLS (Neutral loss scan) and DMRM (Dynamic multiple reaction monitoring). 
Also, we provide variations of these methods including chromatographic 
techniques and different kinds of quantification.