The eukaryotic genome forms a chromatin structure that contains repeating 
nucleosome structures. Nucleosome packaging is regulated by chromatin remodeling 
factors such as histone chaperones. The Saccharomyces cerevisiae H3/H4 histone 
chaperones, CAF-1 and Asf1, regulate DNA replication and chromatin assembly. 
CAF-1 function is largely restricted to non-transcriptional processes in 
heterochromatin, whereas Asf1 regulates transcription together with another 
H3/H4 chaperone, HIR. This study examined the role of the yeast H3/H4 histone 
chaperones, Asf1, HIR, and CAF-1 in chromatin dynamics during transcription. 
Unexpectedly, CAF-1 was recruited to the actively transcribed region in a 
similar way to HIR and Asf1. In addition, the three histone chaperones 
genetically interacted with Set2-dependent H3 K36 methylation. Similar to 
histone chaperones, Set2 was required for tolerance to excess histone H3 but not 
to excess H2A, suggesting that CAF-1, Asf1, HIR, and Set2 function in a related 
pathway and target chromatin during transcription.