A gene-centric Human Proteome Project has been proposed to characterize the 
human protein-coding genes in a chromosome-centered manner to understand human 
biology and disease. Here, we report on the protein evidence for all genes 
predicted from the genome sequence based on manual annotation from literature 
(UniProt), antibody-based profiling in cells, tissues and organs and analysis of 
the transcript profiles using next generation sequencing in human cell lines of 
different origins. We estimate that there is good evidence for protein existence 
for 69% (n = 13985) of the human protein-coding genes, while 23% have only 
evidence on the RNA level and 7% still lack experimental evidence. Analysis of 
the expression patterns shows few tissue-specific proteins and approximately 
half of the genes expressed in all the analyzed cells. The status for each gene 
with regards to protein evidence is visualized in a chromosome-centric manner as 
part of a new version of the Human Protein Atlas ( www.proteinatlas.org ).