OBJECTIVE: The present study evaluates the effect of food intake on 92 
biomarkers for cardiovascular disease (CVD).
METHODS: Twenty two healthy subjects (11 male and 11 female aged 25.9±4.2 years) 
were investigated. A total of 92 biomarkers were measured before a standardized 
meal as well as 30 and 120 minutes afterwards with the Proseek Multiplex CVD III 
kit.
RESULTS: The levels for eight biomarkers decreased significantly (P<0.05) 30 
minutes after food intake. The levels for seven biomarkers remained 
significantly decreased 120 minutes after food intake. Nine biomarker decreased 
significantly at 120 minutes after food intake. The changes were between 4-30%, 
most commonly around 5%. Only six biomarkers showed a difference of 10% or more 
due to food intake. The biggest differences were observed for Insulin-like 
growth factor-binding protein 1 (30%); Azurocidin, Cystatin-B, and 
Myeloperoxidase (13%); Monocyte chemotactic protein 1 (11%); and Myeloblastin 
(10%), all 120 minutes after food intake.
CONCLUSIONS: This study shows that food intake affects several different CVD 
biomarkers, but the effect is predominantly modest. Timing of blood sampling in 
relation to food intake, therefore, appears not to be a major concern. Further 
studies are warranted in older healthy subjects and in patients with various 
cardiac diseases to determine whether the findings are reproducible.