Gas vesicle synthesis in Haloferax mediterranei involves several gene products 
encoded by a 9.4-kilobase pair DNA region (mc-vac region) that contains 13 genes 
in addition to gvpA encoding the major structural gas vesicle protein. The 
expression of part of this region, encompassing the genes gvpA, gvpC, gvpN, and 
gvpO was investigated. These genes are transcribed from a common promoter 
located upstream of gvpA. Transcripts of 0.34 (gvpA only), 1.8 (gvpA/C), 2.4 
(gvpA/C/N) and 3 kilobases (gvpA/C/N/O) were observed, with the gvpA transcript 
being the predominant mRNA species. The majority of the mRNA formed terminates 
64 base pairs downstream of gvpA at the cytosine of the sequence 5' TTTTTC 3'. 
The synthesis of the GvpA and GvpC proteins was investigated by Western 
analyses. An antiserum raised against isolated gas vesicles of Hf. mediterranei 
detects, in addition to gas vesicle fragments, the GvpA protein of the M(r) of 
approximately 8,000 in lysates derived from different halobacteria or from 
Escherichia coli expressing gvpA. In samples containing isolated gas vesicles, 
mainly partially disaggregated gas vesicle fragments hybridize, but a minor 
amount of monomeric GvpA is also seen. For the detection of the GvpC protein, 
two versions of the gvpC gene (full length and gvp delta C lacking the 3' part 
encoding the acidic C terminus) were expressed in E. coli, and the resulting 
proteins were purified. The two antisera raised against these GvpC versions 
indicate the expression of gvpC in different halobacteria. By Western analysis, 
GvpC is also detectable in samples containing isolated gas vesicles 
demonstrating that GvpC is a second, but minor, gas vesicle structural protein.