5-hydroxymethylcytosine (5hmC), an oxidized derivative of 5-methylcytosine 
(5mC), has been implicated as an important epigenetic regulator of mammalian 
development. Current procedures use DNA sequencing methods to discriminate 5hmC 
from 5mC, limiting their accessibility to the scientific community. Here we 
report a method that combines TET-assisted bisulfite conversion with Illumina 
450K DNA methylation arrays for a low-cost high-throughput approach that 
distinguishes 5hmC and 5mC signals at base resolution. Implementing this 
approach, termed "TAB-array", we assessed DNA methylation dynamics in the 
differentiation of human pluripotent stem cells into cardiovascular progenitors 
and neural precursor cells. With the ability to discriminate 5mC and 5hmC, we 
identified a large number of novel dynamically methylated genomic regions that 
are implicated in the development of these lineages. The increased resolution 
and accuracy afforded by this approach provides a powerful means to investigate 
the distinct contributions of 5mC and 5hmC in human development and disease.