Genome-wide Okazaki fragment distribution can differentiate the discontinuous 
from the semi-discontinuous DNA replication model. Here, we investigated the 
genome-wide Okazaki fragment distribution in Saccharomyces cerevisiae S288C. We 
improved the method based upon lambda exonuclease digestion to purify Okazaki 
fragments from S288C yeast cells, followed by Illumina sequencing. The 
distribution of Okazaki fragments around confirmed replication origins, 
including two highly efficient replication origins, supported the discontinuous 
DNA replication model. In S. cerevisiae mitochondria, Okazaki fragments were 
overrepresented in the transcribed regions, indicating the interplay between 
transcription and DNA replication.