DNA replication during S phase is accompanied by establishment of sister 
chromatid cohesion to ensure faithful chromosome segregation. The Eco1 
acetyltransferase, helped by factors including Ctf4 and Chl1, concomitantly 
acetylates the chromosomal cohesin complex to stabilize its cohesive links. Here 
we show that Ctf4 recruits the Chl1 helicase to the replisome via a conserved 
interaction motif that Chl1 shares with GINS and polymerase α. We visualize 
recruitment by EM analysis of a reconstituted Chl1-Ctf4-GINS assembly. The Chl1 
helicase facilitates replication fork progression under conditions of nucleotide 
depletion, partly independently of Ctf4 interaction. Conversely, Ctf4 
interaction, but not helicase activity, is required for Chl1's role in sister 
chromatid cohesion. A physical interaction between Chl1 and the cohesin complex 
during S phase suggests that Chl1 contacts cohesin to facilitate its 
acetylation. Our results reveal how Ctf4 forms a replisomal interaction hub that 
coordinates replication fork progression and sister chromatid cohesion 
establishment.