The Saccharomyces cerevisiae iron-responsive transcription factor, Aft1, has a 
well established role in regulating iron homeostasis through the transcriptional 
induction of iron-regulon genes. However, recent studies have implicated Aft1 in 
other cellular processes independent of iron regulation such as chromosome 
stability. In addition, chromosome spreads and two-hybrid data suggest that Aft1 
interacts with and co-localizes with kinetochore proteins; however, the cellular 
implications of this have not been established. Here, we demonstrate that Aft1 
associates with the kinetochore complex through Iml3. Furthermore, like Iml3, 
Aft1 is required for the increased association of cohesin with pericentric 
chromatin, which is required to resist microtubule tension, and aft1Δ cells 
display chromosome segregation defects in meiosis. Our work defines a new role 
for Aft1 in chromosome stability and transmission.