The functional study of Y chromosome genes has been hindered by a lack of mouse 
models with specific Y chromosome mutations. We used transcription 
activator-like effector nuclease (TALEN)-mediated gene editing in mouse 
embryonic stem cells (mESCs) to produce mice with targeted gene disruptions and 
insertions in two Y-linked genes--Sry and Uty. TALEN-mediated gene editing is a 
useful tool for dissecting the biology of the Y chromosome.