Protein tertiary structure prediction algorithms aim to predict, from amino acid 
sequence, the tertiary structure of a protein. In silico protein structure 
prediction methods have become extremely important, as in vitro-based structural 
elucidation is unable to keep pace with the current growth of sequence databases 
due to high-throughput next-generation sequencing, which has exacerbated the 
gaps in our knowledge between sequences and structures.Here we briefly discuss 
protein tertiary structure prediction, the biennial competition for the Critical 
Assessment of Techniques for Protein Structure Prediction (CASP) and its role in 
shaping the field. We also discuss, in detail, our cutting-edge web-server 
method IntFOLD2-TS for tertiary structure prediction. Furthermore, we provide a 
step-by-step guide on using the IntFOLD2-TS web server, along with some real 
world examples, where the IntFOLD server can and has been used to improve 
protein tertiary structure prediction and aid in functional elucidation.