The ability to adequately measure the phosphorylation state of a protein has 
major biological as well as clinical relevance. Due to its variable nature, 
reversible protein phosphorylations are sensitive to changes in the tissue 
environment. StabilizorTM T1 is a system for rapid inactivation of enzymatic 
activity in biological samples. Enzyme inactivation is accomplished using 
thermal denaturation in a rapid, homogeneous, and reproducible fashion without 
the need of added chemical inhibitors. Using pCREB(Ser133) as a model system, 
the applicability of the Stabilizor system to preserve a rapidly lost 
phosphorylation is shown.