In most eukaryotes, the ribonucleoprotein complex telomerase is responsible for 
maintaining telomere length. In recent years, single-cell microscopy techniques 
such as fluorescent in situ hybridization and live-cell imaging have been 
developed to image the RNA subunit of the telomerase holoenzyme. These 
techniques are now becoming important tools for the study of telomerase 
biogenesis, its association with telomeres and its regulation. Here, we present 
detailed protocols for live-cell imaging of the Saccharomyces cerevisiae 
telomerase RNA subunit, called TLC1, and also of the non-coding telomeric 
repeat-containing RNA TERRA. We describe the approach used for genomic 
integration of MS2 stem-loops in these transcripts, and provide information for 
optimal live-cell imaging of these non-coding RNAs.