Recent studies of animal models have suggested that an increase in the number of 
T cells due to both peripheral expansion and increased thymic T cell output 
plays a key role in the regulation of bone loss after ovariectomy. 
Osteoclastogenic cytokines which are either produced by T cells or activate T 
cells have also been implicated in ovx induced bone loss. Among them are TNF 
alpha and IL-7. The present study investigates the role of thymectomy (THX) and 
IL-7 in bone metabolism in humans. We studied T cells subsets, cytokine 
production and bone metabolism in 13 women thymectomized for Myasthenia gravis 
as compared to healthy controls. Our data demonstrate that the number of CD4+ 
and TNF-producing T cells is lower in THX women as compared to euthymic 
controls. However in THX women the residual T cells produce higher levels of 
IL-7 and RANKL. Furthermore, flow cytometry shows that IL-7 is produced by T and 
B cells. Serum levels of TNF alpha were unaffected by THX and both serum TNF 
alpha and the RANKL/OPG correlated inversely with BMD. There were no differences 
in bone turnover and bone mineral density between THX women and the controls. 
These data suggest that THX decreases the number of TNF-producing CD4+ T cells 
but does not alters serum TNF levels. The RANKL/OPG ratio and indices of bone 
metabolisms are also not affected by THX, although THX increases the levels of 
IL-7 and RANKL. Further studies are needed to clarify the role of thymus in bone 
metabolism and osteoclastogenesis in postmenopausal women.