The recount2 resource is composed of over 70,000 uniformly processed human 
RNA-seq samples spanning TCGA and SRA, including GTEx. The processed data can be 
accessed via the recount2 website and the recount Bioconductor package. This 
workflow explains in detail how to use the recount package and how to integrate 
it with other Bioconductor packages for several analyses that can be carried out 
with the recount2 resource. In particular, we describe how the coverage count 
matrices were computed in recount2 as well as different ways of obtaining public 
metadata, which can facilitate downstream analyses. Step-by-step directions show 
how to do a gene-level differential expression analysis, visualize base-level 
genome coverage data, and perform an analyses at multiple feature levels. This 
workflow thus provides further information to understand the data in recount2 
and a compendium of R code to use the data.