Human toxocariasis which is caused mainly by the larvae of Toxocara canis and 
Toxocara cati, is a worldwide zoonotic disease that can be a potentially serious 
human infection. The enzyme-linked immunosorbent assay (ELISA) using T. canis 
excretory-secretory (TES) antigens harvested from T. canis larvae is currently 
the serological test for confirming toxocariasis. An alternative to producing 
large amounts of Toxocara TES and improved diagnosis for toxocariasis is through 
the development of highly specific recombinant antigens such as the T. canis 
second stage larva excretory-secretory 30 kDa protein (recTES-30). The aim of 
this study was to evaluate the sensitivity and specificity of a rapid diagnostic 
kit (RDT, named as iToxocara kit) in comparison to recTES-30 ELISA in Serendah 
Orang Asli village in Selangor, Malaysia. A total of 133 subjects were included 
in the study. The overall prevalence rates by ELISA and RDT were 29.3% and 
33.1%, respectively, with more positive cases detected in males than females. 
However, no association was found between toxocariasis and gender or age. The 
percentage sensitivity, specificity, positive predictive value and negative 
predictive value of RDT were 85.7%, 90.1%, 80% and 93.2%, respectively. The 
prevalence for toxocariasis in this population using both ELISA and RDT was 
27.1% (36/133) and the K-concordance test suggested good agreement of the two 
tests with a Cohen's kappa of 0.722, P<0.01. In addition, the followed-up 
Spearman rank correlation showed a moderately high correlation at R=0.704 and 
P<0.01. In conclusion, the RDT kit was faster and easier to use than an ELISA 
and is useful for the laboratory diagnosis of hospitalized cases of 
toxocariasis.