Adenoviral vectors have proven to be valuable resources in the development of 
novel therapies aimed at targeting pathological conditions of the central 
nervous system, including Alzheimer's disease and neoplastic brain lesions. Not 
only can some genetically engineered adenoviral vectors achieve remarkably 
efficient and specific gene delivery to target cells, but they also may act as 
anticancer agents by selectively replicating within cancer cells.Due to the 
great interest in using adenoviral vectors for various purposes, the need for a 
comprehensive protocol for viral vector production is especially apparent. Here, 
we describe the process of generating an adenoviral vector in its entirety, 
including the more complex process of adenoviral fiber modification to restrict 
viral tropism in order to achieve more efficient and specific gene delivery.