Technology development has always been one of the forces driving breakthroughs 
in biomedical research. Since the time of Thomas Morgan, Drosophilists have, 
step by step, developed powerful genetic tools for manipulating and functionally 
dissecting the Drosophila genome, but room for improving these technologies and 
developing new techniques is still large, especially today as biologists start 
to study systematically the functional genomics of different model organisms, 
including humans, in a high-throughput manner. Here, we report, for the first 
time in Drosophila, a rapid, easy, and highly specific method for modifying the 
Drosophila genome at a very high efficiency by means of an improved 
transcription activator-like effector nuclease (TALEN) strategy. We took 
advantage of the very recently developed "unit assembly" strategy to assemble 
two pairs of specific TALENs designed to modify the yellow gene (on the sex 
chromosome) and a novel autosomal gene. The mRNAs of TALENs were subsequently 
injected into Drosophila embryos. From 31.2% of the injected F(0) fertile flies, 
we detected inheritable modification involving the yellow gene. The entire 
process from construction of specific TALENs to detection of inheritable 
modifications can be accomplished within one month. The potential applications 
of this TALEN-mediated genome modification method in Drosophila are discussed.