Oncogene-induced senescence (OIS) is a highly dynamic process, involving several 
different effector mechanisms, the multitude and combination of which likely 
determines the quality of the phenotype (Pérez-Mancera et al., Nat Rev Cancer 
14:547-558, 2014). Autophagy, a cellular degradation process, has been proposed 
to be one of these senescence effectors, although its functional relevance seems 
highly context dependent (Hoare et al., Semin Cancer Biol 21:397-404, 2011). A 
number of methods for monitoring autophagy are available, and several excellent 
protocols have been published in this journal (Klionsky et al., Autophagy 
8:445-544, 2012; Tooze et al., Methods Mol Biol 1270:155-165, 2015; Tabata et 
al., Methods Mol Biol 931:449-466, 2013; Young and Tooze, Methods Mol Biol 
445:147-157, 2008). The same principles apply to models of OIS in culture. Thus, 
in this chapter, we describe how to generate OIS cells using human diploid 
fibroblasts (HDFs), the best-characterized cell model of OIS, and how to detect 
autophagy, particularly focusing on immunofluorescence methods.