Two-color flow-cytometric analysis on peripheral blood lymphocytes of 46 
untreated multiple sclerosis patients (MS), 36 other medical disease patients 
(OMD) and 19 healthy control subjects (HC) was performed to know the 
relationships between T and B cell subpopulations. In MS patients we observed an 
increase of total lymphocyte count and an increase of CD4+CD29+ cells, which are 
adjuvant to B cell in antibody production. We hypothesized this change is 
related to the reduction of CD21+ cells, expressing B2 antigen which disappears 
after B cell activation. The unperfect balance of immune system in MS was also 
demonstrated by the increased level of CD25+ cells in relapsing-remitting 
patients and by the decreased level of CD4+ CD45RA+ (suppressor inducer) cells 
in progressive patients.