Cattle botulism is a fatal intoxication caused by botulinum neurotoxins (BoNTs) 
produced by Clostridium botulinum serotypes C and D resulting in economic 
losses. Vaccination is the most effective way to control botulism. However, the 
commercially available vaccines are difficult and hazardous to produce. 
Neutralizing antibodies against the C-terminal fragment of the BoNT heavy chain 
(HC) are known to protect against lethal doses of BoNTs. We report the 
vaccination of cattle with a previously tested recombinant chimera consisting of 
Escherichia coli heat-labile enterotoxin B subunit and the HC of BoNTs C and D. 
Vaccinated animals produced neutralizing antibodies against serotypes C and D 
averaging 5±0 and 6.14±1.06IU/mL, respectively. For BoNT D, the titers were 
greater than those measured for the commercial vaccine, which induced titers of 
5±0 and 2.85±1.35 against the respective serotypes, suggesting that this chimera 
is effective against cattle botulism.