Growth differentiation factor 10 (Gdf10), also known as Bmp3b, is a member of 
the transforming growth factor (TGF)-ß superfamily. Gdf10 is expressed in 
Bergmann glial cells, which was investigated by single-cell transcriptional 
profiling (Koirala and Corfas, (2010) PLoS ONE 5: e9198). Here we provide a 
detailed characterization of Gdf10 expression from E14, the stage at which Gdf10 
is expressed for the first time in the cerebellum, until P28. We detected Gdf10 
expression in both germinal zones: in the ventricular zone (VZ) of the 4th 
ventricle as well as in the rhombic lip (RL). The VZ has been postulated to give 
rise to GABAergic neurons and glial cells, whereas the RL gives rise to 
glutamatergic neurons. Thus, it was very surprising to discover a gene that is 
expressed exclusively in glial cells and is not restricted to an expression in 
the VZ, but is also present in the RL. At postnatal stages Gdf10 was distributed 
equally in Bergmann glial cells of the cerebellum. Furthermore, we found Gdf10 
to be regulated by Sonic hedgehog (Shh), which is secreted by Purkinje cells of 
the cerebellum. In the conditional Shh mutants, glial cells showed a reduced 
expression of Gdf10, whereas the expression of Nestin and Vimentin was 
unchanged. Thus, we show for the first time, that Gdf10, expressed in Bergmann 
glial cells, is affected by the loss of Shh as early as E18.5, suggesting a 
regulation of glial development by Shh.