Mast cells (MCs) are known to participate in a variety of patho-physiological 
processes depending largely on the intragranular mediators and the production of 
cytokines and chemokines during degranulation. Recently, extracellular vesicles 
(EVs) have been implicated important functions for MCs, but the components of 
MC-derived EVs have not yet been well-characterized. In this study, we aimed to 
identify signatures of proteins, long non-coding RNAs (lncRNAs), and microRNAs 
(miRNAs) in EVs derived from resting (Rest-EV) and degranulated (Sti-EV) MCs by 
differential ultracentrifugation. Using tandem mass tag (TMT)-based quantitative 
proteomics technology and RNA sequencing, we identified a total of 1988 
proteins, 397 lncRNAs, and 272 miRNAs in Rest-EV and Sti-EV. The proteins 
include common EVs markers (cytoskeletal proteins), MCs markers (FcεRI and 
tryptase), and some preformed MCs mediators (lysosomal enzymes) as well. The 
global expression profiles of lncRNAs and miRNAs identified, for the first time, 
from Rest-EV and Sti-EV, strongly suggest a potential regulatory function of 
MC-derived EVs. We have also performed Western blotting and qRT-PCR analysis to 
further verify some of the proteins, lncRNAs, and miRNAs identified from Rest-EV 
and Sti-EV. Our findings will help to elucidate the functions of MC-derived EVs, 
and provide a reference dataset for future translational studies involving 
MC-derived EVs.