The rat whisker hair follicle (HF) is a model for studying the reconstruction of 
the HF or dermal papilla (DP), and involves the Wnt/β-catenin signaling pathway, 
which is a key pathway in HF development and HF cycling after birth. It has been 
reported that Wnt/catenin signaling plays an indispensable role in human or rat 
pelages development and postnatal growth. However, the distribution of some 
Wnt/β-catenin signaling pathway factors and their relationship with the 
epithelial stem cell markers in whisker follicles has not been characterized. In 
this study, we investigated the immunolocalization of Wnt/catenin signaling 
pathway members, including Wnt10b, Wnt10a, Wnt5a, β-catenin, and downstream 
lymphoid enhancer-binding factor 1 (LEF1) and transcription factor 3 (TCF3), as 
well as, HF stem-cell markers CD34, CK15 and proliferating cell nuclear antigen 
(PCNA) protein, in rat anagen phase whisker follicles. β-catenin, Wnt5a, Wnt10b, 
Wnt10a, LEF1, and TCF3 were expressed in the outer root sheath (ORS), inner root 
sheath, matrix and hair shaft of anagen follicles. β-catenin, Wnt10b, LEF1, and 
TCF3 were highly expressed and Wnt5a and Wnt10a weakly expressed in DP and 
dermal sheath (DS) regions. The expression of α-smooth muscle actin was strong 
in the lower DS and it was also detected in some DP cells. CD34, CK15 and PCNA 
were all expressed in the ORS; and CD34 and PCNA were also detected in the 
matrix, however CD34 was extensively expressed in DP and DS regions. Our studies 
located the position of Wnts, downstream LEF1 and TCF3 and stem cell marker 
proteins, which provide new information in understanding the role of the Wnt 
singaling pathway in whisker follicles' growth.